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產(chǎn)品展示 / products 您的位置:網(wǎng)站首頁(yè) > 產(chǎn)品展示 > TwistDx恒溫?cái)U(kuò)增 > TWISTDX TwistAmp NFO kits > TwistAmp NFOTWISTDX TwistAmp NFO kits
TWISTDX TwistAmp NFO kits

TWISTDX TwistAmp NFO kits

產(chǎn)品型號(hào): TwistAmp NFO

所屬分類:TWISTDX TwistAmp NFO kits

產(chǎn)品時(shí)間:2020-06-09

簡(jiǎn)要描述:TwistAmp® 核酸擴(kuò)增產(chǎn)品,能夠在15分鐘內(nèi)進(jìn)行常溫下的單分子核酸檢測(cè)該技術(shù)對(duì)硬件設(shè)備的要求很低,特別適合用于體外診斷、獸醫(yī)、食品安全、生物安全、農(nóng)業(yè)等領(lǐng)域。TwistAmp nfo用于末端檢測(cè),檢測(cè)DNA擴(kuò)增反應(yīng). 末端三明治法快速檢測(cè),如側(cè)向流試紙法.除基本組分之外, nfo核酶,和適合是LF 探針 形成新的聚合酶增強(qiáng)底物,無(wú)需任何儀器。本品可通過(guò)加入exo探針作為Amp exo kit

詳情介紹

TWISTDX TwistAmp NFO kits

TwistDx品牌國(guó)內(nèi)代理商--默瑞(上海)生物科技有限公司

TwistAmp® 核酸擴(kuò)增產(chǎn)品,能夠在15分鐘內(nèi)進(jìn)行常溫下的單分子核酸檢測(cè)該技術(shù)對(duì)硬件設(shè)備的要求很低,特別適合用于體外診斷、獸醫(yī)、食品安全、生物安全、農(nóng)業(yè)等領(lǐng)域。TwistAmp nfo用于末端檢測(cè),檢測(cè)DNA擴(kuò)增反應(yīng). 末端三明治法快速檢測(cè),如側(cè)向流試紙法.除基本組分之外, nfo核酶,和適合是LF 探針 形成新的聚合酶增強(qiáng)底物,無(wú)需任何儀器。本品可通過(guò)加入exo探針作為Amp exo kit的替代物,進(jìn)行熒光定量檢測(cè),由于核酶延緩動(dòng)態(tài)反應(yīng)速度,可用于末端電泳檢測(cè)。

TwistAmp® nfo

產(chǎn)品貨號(hào)-TANFO02KIT

產(chǎn)品貨號(hào)-TwistAmp® nfo

產(chǎn)品用途-用于末端檢測(cè),檢測(cè) DNA 復(fù)制反應(yīng)

配套儀器-Twirla

TWISTDX TwistAmp NFO kits假陽(yáng)性問(wèn)題解答

Q:What will cause the false-positive results except DNA contamination?

A:For the nfo kits another source of false positives is the dimerization of the two primers and in some cases the labeled primer, and the probe.

When this happens you will have the biotin and FITC on an RPA product that will bind to the Lateral flow strip and bind to the ant-FITC gold particle that will result in a line in the test area on the Lateral Flow Strip.  

The primer sequences and probe sequence should be check for possible dimerization and they may need to be modified and screened.

There are some basic rules for PCR primers that also hold true for RPA primers. Namely try to avoid:

1) Long homo-polymer repeats

2) Repetitive sequences

3) Regions that could have lots of secondary structure

4) High (>70%) GC or AT regions

5) Sequences likely to cause primer-primer interaction (use 

產(chǎn)品簡(jiǎn)介

TwistAmp® 核酸擴(kuò)增產(chǎn)品,能夠在15分鐘內(nèi)進(jìn)行常溫下的單分子核酸檢測(cè)該技術(shù)對(duì)硬件設(shè)備的要求很低,特別適合用于體外診斷、獸醫(yī)、食品安全、生物安全、農(nóng)業(yè)等領(lǐng)域。TwistAmp exo 用于real-time探針檢測(cè). 除基本組分外, 還含有內(nèi)切酶3,減少擴(kuò)增產(chǎn)物的末端拷貝量,所以不能用于電泳分析,但在RPA系統(tǒng)中產(chǎn)生更強(qiáng)的動(dòng)態(tài)熒光信號(hào),便于實(shí)時(shí)定量。

產(chǎn)品詳情介紹

默瑞生物

默瑞(上海)生物科技有限公司,作為專業(yè)的生命科學(xué)產(chǎn)品推廣與服務(wù)商,以“創(chuàng)新,專業(yè),共贏”的理念,不斷強(qiáng)化服務(wù)體系,提升客戶體驗(yàn),以莫納生物產(chǎn)品為主線,結(jié)合國(guó)內(nèi)外優(yōu)質(zhì)產(chǎn)品提供高標(biāo)準(zhǔn)完整解決方案,幫助科研客戶和生物技術(shù)企業(yè)高效創(chuàng)新,加速轉(zhuǎn)化,創(chuàng)造更多優(yōu)質(zhì)產(chǎn)品,提升社會(huì)福祉,實(shí)現(xiàn)多方共贏

默瑞生物創(chuàng)立以來(lái),已經(jīng)陸續(xù)導(dǎo)入:

TwistDx,Immunostep,101Bio,enzymatics,AATBioquest,KAPABIOSYSTEMS,BioDynami,Jackson,Echelon,ChromoTek,Nanocs,Biotechrabbit,LeeBiosolutions, magtivio等優(yōu)質(zhì)品牌,逐步培育成為專業(yè)用戶的選擇。



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