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技術(shù)文章 / article 您的位置:網(wǎng)站首頁 > 技術(shù)文章 > 作為中國PCR行業(yè)--核酸純化試劑(磁珠法)

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作為中國PCR行業(yè)--核酸純化試劑(磁珠法)

發(fā)布時間: 2021-04-07  點擊次數(shù): 3508次

博日作為中國PCR行業(yè),公司已開發(fā)并量產(chǎn)實時熒光定量PCR分析儀、全自動核酸純化儀、基因擴增儀、核酸純化試劑、各類PCR檢測試劑及樣本保存液等多類產(chǎn)品。銷售網(wǎng)絡遍布121個國家和地區(qū)。此外,公司全新投資建設(shè)現(xiàn)代化耗材生產(chǎn)基地,研發(fā)和生產(chǎn)分子診斷耗材產(chǎn)品,進一步打造博日科技“儀器+試劑+耗材”的全產(chǎn)業(yè)鏈經(jīng)營模式,大力推動中國PCR產(chǎn)業(yè)的進口替代發(fā)展進程。

磁珠法核酸提取簡介

磁珠法核酸提取,具有傳統(tǒng)DNA提取方法*的優(yōu)勢,主要體現(xiàn)在 :

①能夠?qū)崿F(xiàn)自動化、大批量操作,目前已有96孔的核酸自動提取儀,用一個樣品的提取時間即可實現(xiàn)對96個樣品的處理,符合生物學高通量的操作要求,使得傳染性疾病爆發(fā)時能夠進行快速及時的應對,這一特點使得傳統(tǒng)方法*;

②操作簡單、用時短,整個提取流程只有四步,大多可以在36-40分鐘內(nèi)完成;

③安全無毒,不使用傳統(tǒng)方法中的苯、有毒試劑,對實驗操作人員的傷害減少到最少,*符合現(xiàn)代環(huán)保理念;

④磁珠與核酸的特異性結(jié)合使得提取的核酸純度高、濃度大。

磁珠法核酸提取原理

依據(jù)與硅膠膜離心柱相同的原理 ,運用納米技術(shù)對超順磁性納米顆粒的表面進行改良和表面修飾后,制備成超順磁性氧化硅納米磁珠。該磁珠能在微觀界面上與核酸分子特異性地識別和高效結(jié)合。利用二氧化硅包被的納米磁性微球的超順磁性,在Chaotropic鹽(鹽酸胍、異硫氰酸胍等)和外加磁場的作用下,能從血液、動物組織、食品、病原微生物等樣本中分離出DNA和RNA,可應用在臨床疾病診斷、輸血安全、法醫(yī)學鑒定、環(huán)境微生物檢測、食品安全檢測、分子生物學研究等多種領(lǐng)域。

磁珠法核酸提取過程

磁珠法核酸提取一般可以分為四步:裂解——結(jié)合——洗滌——洗脫。

1592888436_5352.jpg

MagaBio plus病毒DNA/RNA純化試劑盒Ⅱ

產(chǎn)品詳情:

MagaBio plus病毒DNA/RNA純化試劑盒Ⅱ是專門用于從全血、血清、血漿、病料、采樣拭子、糞便和體液等樣本中提取高純度的病毒DNA/RNA。本試劑盒采用了*的裂解液系統(tǒng),高效快速的裂解病毒釋放出DNA或RNA,再結(jié)合新型的納米級磁珠技術(shù)高效純化核酸。本試劑盒具有穩(wěn)定性好、操作簡單、靈敏度高等特點,整個提取過程僅需35min。純化后的核酸可直接用于下游如NGS,qPCR等檢測。

產(chǎn)品特點:

(1)適用性強:適用于各種DNA和RNA病毒核酸的提取

(2)樣本廣泛:可輕松提取全血、血漿、血清、病料、采樣拭子、糞便和體液等中的病毒核酸   

(3)高效快速:一次性提取高達96個樣本僅需35分鐘

(4)靈敏度高:DNA病毒可以達到10 IU/mL,RNA病毒可以達到50 IU/mL 

(5)穩(wěn)定可靠:試劑配套自動化儀器,使檢測結(jié)果更加穩(wěn)定可靠    

 (6)安全性高:無酚、有毒試劑

應用案例

1592888436_5352.jpg

核酸純化試劑(磁珠法)

BSC71S1B MagaBio plus病毒DNA/RNA純化試劑盒Ⅱ

BSC53S1B MagaBio plus總RNA純化試劑盒

BSC37S1C MagaBio Plus組織基因組DNA純化試劑盒

BSC57S1B MagaBio Plus病毒DNA/RNA純化試劑盒

BSC58S1B MagaBio Plus病毒RNA純化試劑盒

BSC11S1B MagaBio Plus病毒DNA純化試劑盒

BSC09S1B MagaBio Plus 細菌基因組DNA純化試劑盒

BSC10S1B MagaBio Plus 植物組DNA純化試劑盒

BSC08S1B MagaBio plus 全血基因組DNA純化試劑盒

BSC07S1B MagaBio plus 通用基因組DNA純化試劑盒

BSC40S1 Magabio血漿游離DNA提取試劑盒

BSC35S1 MagaBio口腔拭子基因組DNA 純化試劑盒

BSC34S1 MagaBio干血斑基因組DNA 純化試劑盒

BSC33S1 MagaBio 昆蟲基因組DNA純化試劑盒

BSC31S1 MagaBio 石蠟包埋組織基因組DNA純化試劑盒

默瑞生物

默瑞(上海)生物科技有限公司,作為專業(yè)的生命科學產(chǎn)品推廣與服務商,以“創(chuàng)新,專業(yè),共贏”的理念,不斷強化服務體系,提升客戶體驗,結(jié)合國內(nèi)外優(yōu)質(zhì)產(chǎn)品提供高標準完整解決方案,幫助科研客戶和生物技術(shù)企業(yè)高效創(chuàng)新,加速轉(zhuǎn)化,創(chuàng)造更多優(yōu)質(zhì)產(chǎn)品,提升社會福祉,實現(xiàn)多方共贏。

默瑞生物創(chuàng)立以來,已經(jīng)陸續(xù)導入TwistDx,BACPAC,博日,Celexplorer

DiscoverX,Canvax,Jackson,izon,Biontex,Immunostep,101Bio,enzymatics,AATBioquest,KAPABIOSYSTEMS,BioDynami,Echelon,ChromoTek,Nanocs,Biotechrabbit,LeeBiosolutions, magtivio等優(yōu)質(zhì)國際品牌,逐步培育成為專業(yè)用戶的選擇。

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